› 1fyk

X-ray diffraction
2.5Å resolution

SERENDIPITOUS CRYSTAL STRUCTURE CONTAINING THE HEAT SHOCK TRANSCRIPTION FACTOR'S DNA BINDING DOMAIN AND COGNATE DNA THAT IS TRANSLATIONALLY DISORDERED

Released:
DOI: 10.2210/pdb1fyk/pdb
PDB entry 1fyk coloured by chain, front view.
PDB entry 1fyk coloured by chain, side view.
PDB entry 1fyk coloured by chain, top view.
Source organism: Kluyveromyces lactis
Primary publication:
Crystal packing interaction that blocks crystallization of a site-specific DNA binding protein-DNA complex.
Littlefield O, Nelson HC
Proteins 45 219-28 (2001)
PMID: 11599025

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113828 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Heat shock transcription factor Chain: A
Molecule details ›
Chain: A
Length: 92 amino acids
Theoretical weight: 11.09 KDa
Source organism: Kluyveromyces lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: P22121 (Residues: 193-284; Coverage: 14%)
Gene names: HSF, KLLA0D03322g
Sequence domains: HSF-type DNA-binding
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
HSE DNA-PHOSPHATE BACKBONE Chain: C
Molecule details ›
Chain: C
Length: 4 nucleotides
Theoretical weight: 739 Da
Source organism: Kluyveromyces lactis
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: C2
Unit cell:
a: 103.15Å b: 32.99Å c: 42.57Å
α: 90° β: 109.9° γ: 90°
R-values:
R R work R free
0.209 0.209 0.278
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 citation in other articles

De novo appearance and "strain" formation of yeast prion [PSI+] are regulated by the heat-shock transcription factor.
Park et al. (2006)
1 more