› 1fv1

X-ray diffraction
1.9Å resolution

STRUCTURAL BASIS FOR THE BINDING OF AN IMMUNODOMINANT PEPTIDE FROM MYELIN BASIC PROTEIN IN DIFFERENT REGISTERS BY TWO HLA-DR2 ALLELES

Released:
DOI: 10.2210/pdb1fv1/pdb
PDB entry 1fv1 coloured by chain, front view.
PDB entry 1fv1 coloured by chain, side view.
PDB entry 1fv1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the binding of an immunodominant peptide from myelin basic protein in different registers by two HLA-DR2 proteins.
Li Y, Li H, Martin R, Mariuzza RA
J. Mol. Biol. 304 177-88 (2000)
PMID: 11080454

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135349 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class II histocompatibility antigen, DR alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 181 amino acids
Theoretical weight: 21.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01903 (Residues: 26-206; Coverage: 79%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DR beta 5 chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 190 amino acids
Theoretical weight: 22.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q30154 (Residues: 30-219; Coverage: 80%)
Gene name: HLA-DRB5
Sequence domains:
Structure domains:
Myelin basic protein Chains: C, F
Molecule details ›
Chains: C, F
Length: 20 amino acids
Theoretical weight: 2.28 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P02686 (Residues: 218-237; Coverage: 7%)
Gene name: MBP

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P21
Unit cell:
a: 63.189Å b: 114.892Å c: 63.175Å
α: 90° β: 90.9° γ: 90°
R-values:
R R work R free
0.233 0.233 0.267
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

18 review citations

MHC class II proteins and disease: a structural perspective.
Jones et al. (2006)
17 more

36 mentions without citation

Conformational variation in structures of classical and non-classical MHCII proteins and functional implications.
Painter et al. (2012)
35 more