1fok Summary



The structure was published by Wah, D.A., Hirsch, J.A., Dorner, L.F., Schildkraut, I., and Aggarwal, A.K., in 1997 in a paper entitled "Structure of the multimodular endonuclease FokI bound to DNA." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.8 Å and deposited in 1997.

The experimental data on which the structure is based was not deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely DNA (5'-D(*TP*CP*GP*GP*AP*TP*GP*AP*TP*AP*AP*CP*GP*CP*TP*AP*G P*TP*CP*A)-3'), DNA (5'-D(*AP*TP*GP*AP*CP*TP*AP*GP*CP*GP*TP*TP*AP*TP*CP*AP*T P*CP*CP*G)-3'), and PROTEIN (FOKI RESTRICTION ENDONUCLEASE).

The molecule most likely forms heterotrimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A PROTEIN (FOKI RESTRICTION ENDONUCLEASE) P14870 (8-583) (T2F1_PLAOK)search Planomicrobium okeanokoitessearch 99% 576 98%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P14870 (8 - 583) PROTEIN (FOKI RESTRICTION ENDONUCLEASE) Planomicrobium okeanokoites

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A (P14870) Restriction endonuclease FokI, N-terminal (recognition) domainsearch, Restriction endonuclease FokI, C-terminal (catalytic) domainsearch Foki Restriction Endonuclease, Chain A, domain 1search, "winged helix" repressor DNA binding domainsearch, Restriction Endonucleasesearch PF02980: Restriction endonuclease FokI, catalytic domainsearch, PF02981: Restriction endonuclease FokI, recognition domainsearch, PF09254: Restriction endonuclease FokI, C terminalsearch

Chain ID Biological process (GO) Molecular function (GO)
A (P14870) DNA restriction-modification systemsearch nucleic acid phosphodiester bond hydrolysissearch DNA catabolic process, endonucleolyticsearch DNA catabolic processsearch deoxyribonuclease activitysearch Type II site-specific deoxyribonuclease activitysearch endonuclease activitysearch DNA bindingsearch nuclease activitysearch hydrolase activitysearch

Chain InterPro annotation
A Restriction endonuclease, type II, FokI, catalytic domainsearch Restriction endonuclease, type II, FokI, N-terminalsearch Restriction endonuclease type II-likesearch Restriction endonuclease, FokI, C-terminal/endonuclease I, coresearch Winged helix-turn-helix DNA-binding domainsearch Restriction endonuclease, type II, FokI, C-terminalsearch