PDBe Entry: 1fmo

CRYSTAL STRUCTURE OF A POLYHISTIDINE-TAGGED RECOMBINANT CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE COMPLEXED WITH THE PEPTIDE INHIBITOR PKI(5-24) AND ADENOSINE

Summary

Header

COMPLEX (PHOSPHOTRANSFERASE/INHIBITOR)

Method

X-RAY DIFFRACTION

Experiment

Resolution: 2.2 Å, R-factor: 18.2%, Spacegroup: P 21 21 21

Released

14/01/1998, deposition: 08/07/1997, last revision: 24/02/2009

Authors

Narayana, N.

; Cox, S.

; Shaltiel, S.

; Taylor, S.S.

; Xuong, N.-H.

Primary citation

Crystal structure of a polyhistidine-tagged recombinant catalytic subunit of cAMP-dependent protein kinase complexed with the peptide inhibitor PKI(5-24) and adenosine.
BIOCHEMISTRY

vol:36, pag:4438-4448 (1997) [PubMed ID 9109651 ]

Keywords

COMPLEX (PHOSPHOTRANSFERASE/INHIBITOR)

, PHOSPHORYLATION

, POLYHISTIDINE-TAG

, PROTEIN KINASE

2.7.11.11 ExPASy BRENDA

(E)

Organism

Mus musculus(house mouse) 10090

(E)

UniProt

cAMP-dependent protein kinase catalytic subunit alpha (EC 2.7.11.11) (PKA C-alpha) P05132

(E)
cAMP-dependent protein kinase inhibitor alpha (cAMP-dependent protein kinase inhibitor, muscle/brain isoform) (PKI-alpha) P61926

(I)

Solvent

E, I

Polymers

Id	Name	Type	UniProt	Residues	Observed
E	CAMP-DEPENDENT PROTEIN KINASE	Protein	P05132 (KAPCA_MOUSE)	350	96%
I	HEAT STABLE RABBIT SKELETAL MUSCLE INHIBITOR PROTEIN	Protein	P61926 (IPKA_RABIT)	20	100%

Heterogens

Id	Name	Ligands
E	ADENOSINE	ADN