› 1fm8

X-ray diffraction
2.3Å resolution

CHALCONE ISOMERASE COMPLEXED WITH 5,4'-DIDEOXYFLAVANONE

Released:
DOI: 10.2210/pdb1fm8/pdb
PDB entry 1fm8 coloured by chain, front view.
PDB entry 1fm8 coloured by chain, side view.
PDB entry 1fm8 coloured by chain, top view.
Source organism: Medicago sativa
Primary publication:
Reaction mechanism of chalcone isomerase. pH dependence, diffusion control, and product binding differences.
Jez JM, Noel JP
J Biol Chem 277 1361-9 (2002)
PMID: 11698411

Function and Biology Details

Reaction catalysed: 
A chalcone = a flavanone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-151152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone--flavanone isomerase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 23.85 KDa
Source organism: Medicago sativa
Expression system: Escherichia coli
UniProt:
  • Canonical: P28012 (Residues: 1-222; Coverage: 100%)
Gene names: CHI-1, CHI1
Sequence domains: Chalcone-flavanone isomerase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand DDC 1 x DDC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P6522
Unit cell:
a: 89.848Å b: 89.848Å c: 352.956Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.238 0.266
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Biosynthesis of flavonoids and effects of stress.
Winkel-Shirley B. (2002)
17 more

3 mentions without citation

Structure and function of enzymes involved in the biosynthesis of phenylpropanoids.
Ferrer et al. (2008)
2 more