› 1fm6

X-ray diffraction
2.1Å resolution

THE 2.1 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF THE HETERODIMER OF THE HUMAN RXRALPHA AND PPARGAMMA LIGAND BINDING DOMAINS RESPECTIVELY BOUND WITH 9-CIS RETINOIC ACID AND ROSIGLITAZONE AND CO-ACTIVATOR PEPTIDES.

Released:
DOI: 10.2210/pdb1fm6/pdb
PDB entry 1fm6 coloured by chain, front view.
PDB entry 1fm6 coloured by chain, side view.
PDB entry 1fm6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Asymmetry in the PPARgamma/RXRalpha crystal structure reveals the molecular basis of heterodimerization among nuclear receptors.
Gampe RT, Montana VG, Lambert MH, Miller AB, Bledsoe RK, Milburn MV, Kliewer SA, Willson TM, Xu HE
Mol Cell 5 545-55 (2000)
PMID: 10882139

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-148713 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Retinoic acid receptor RXR-alpha Chains: A, U
Molecule details ›
Chains: A, U
Length: 238 amino acids
Theoretical weight: 26.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19793 (Residues: 225-462; Coverage: 52%)
Gene names: NR2B1, RXRA
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Peroxisome proliferator-activated receptor gamma Chains: D, X
Molecule details ›
Chains: D, X
Length: 272 amino acids
Theoretical weight: 31.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37231 (Residues: 234-505; Coverage: 54%)
Gene names: NR1C3, PPARG
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 1 Chains: B, E, V, Y
Molecule details ›
Chains: B, E, V, Y
Length: 25 amino acids
Theoretical weight: 2.81 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15788 (Residues: 676-700; Coverage: 2%)
Gene names: BHLHE74, NCOA1, SRC1
Sequence domains: Steroid receptor coactivator

Ligands and Environments

2 bound ligands:
Ligand 9CR 2 x 9CR
Ligand BRL 2 x BRL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 49.255Å b: 222.364Å c: 55.289Å
α: 90° β: 98.59° γ: 90°
R-values:
R R work R free
0.25 0.25 0.292
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

64 review citations

Fat and beyond: the diverse biology of PPARgamma.
Tontonoz et al. (2008)
63 more

59 mentions without citation

The retinoid X receptors and their ligands.
Dawson et al. (2012)
58 more