› 1flz

X-ray diffraction
2.3Å resolution

URACIL DNA GLYCOSYLASE WITH UAAP

Released:
DOI: 10.2210/pdb1flz/pdb
PDB entry 1flz coloured by chain, front view.
PDB entry 1flz coloured by chain, side view.
PDB entry 1flz coloured by chain, top view.
Source organism: Escherichia coli B
Primary publication:
Stressing-out DNA? The contribution of serine-phosphodiester interactions in catalysis by uracil DNA glycosylase.
Werner RM, Jiang YL, Gordley RG, Jagadeesh GJ, Ladner JE, Xiao G, Tordova M, Gilliland GL, Stivers JT
Biochemistry 39 12585-94 (2000)
PMID: 11027138

Function and Biology Details

Reaction catalysed: 
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 25.55 KDa
Source organism: Escherichia coli B
Expression system: Escherichia coli
UniProt:
  • Canonical: P12295 (Residues: 2-229; Coverage: 100%)
Gene names: JW2564, b2580, ung
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

1 bound ligand:
Ligand URA 1 x URA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P43212
Unit cell:
a: 78.21Å b: 78.21Å c: 80.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 not available not available
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Uracil in DNA--occurrence, consequences and repair.
Krokan et al. (2002)
4 more

8 mentions without citation

Uracil-DNA glycosylases-structural and functional perspectives on an essential family of DNA repair enzymes.
Schormann et al. (2014)
7 more