› 1fjl

X-ray diffraction
2Å resolution

HOMEODOMAIN FROM THE DROSOPHILA PAIRED PROTEIN BOUND TO A DNA OLIGONUCLEOTIDE

Released:
DOI: 10.2210/pdb1fjl/pdb
PDB entry 1fjl coloured by chain, front view.
PDB entry 1fjl coloured by chain, side view.
PDB entry 1fjl coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
High resolution crystal structure of a paired (Pax) class cooperative homeodomain dimer on DNA.
Wilson DS, Guenther B, Desplan C, Kuriyan J
Cell 82 709-19 (1995)
PMID: 7671301

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113808 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Segmentation protein paired Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 81 amino acids
Theoretical weight: 9.72 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P06601 (Residues: 196-276; Coverage: 13%)
Gene names: CG6716, prd
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(*AP*AP*TP*AP*AP*TP*CP*TP*GP*AP*TP*TP*AP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 14 nucleotides
Theoretical weight: 4.26 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
DNA (5'-D(*TP*GP*TP*AP*AP*TP*CP*AP*GP*AP*TP*TP*AP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 14 nucleotides
Theoretical weight: 4.29 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
DNA (5'-D(*TP*GP*TP*AP*AP*TP*CP*TP*GP*AP*TP*TP*AP*C)-3') Chain: F
Molecule details ›
Chain: F
Length: 14 nucleotides
Theoretical weight: 4.27 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 72.21Å b: 146.89Å c: 77.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

31 review citations

PAX-6 in development and evolution.
Callaerts et al. (1997)
30 more

31 mentions without citation

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
30 more