› 1fiq

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF XANTHINE OXIDASE FROM BOVINE MILK

Released:
DOI: 10.2210/pdb1fiq/pdb
PDB entry 1fiq coloured by chain, front view.
PDB entry 1fiq coloured by chain, side view.
PDB entry 1fiq coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structures of bovine milk xanthine dehydrogenase and xanthine oxidase: structure-based mechanism of conversion.
Enroth C, Eger BT, Okamoto K, Nishino T, Nishino T, Pai EF
Proc Natl Acad Sci U S A 97 10723-8 (2000)
PMID: 11005854

Function and Biology Details

Reactions catalysed: 
Xanthine + NAD(+) + H(2)O = urate + NADH
Xanthine + H(2)O + O(2) = urate + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160414 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Xanthine dehydrogenase/oxidase Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 24.04 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P80457 (Residues: 1-219; Coverage: 16%)
Gene name: XDH
Sequence domains:
Structure domains:
Xanthine dehydrogenase/oxidase Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 39.03 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P80457 (Residues: 220-569; Coverage: 26%)
Gene name: XDH
Sequence domains:
Structure domains:
Xanthine dehydrogenase/oxidase Chain: C
Molecule details ›
Chain: C
Length: 763 amino acids
Theoretical weight: 83.96 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P80457 (Residues: 570-1332; Coverage: 57%)
Gene name: XDH
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand MTE 1 x MTE

Cofactor: Ligand FAD 1 x FAD
4 bound ligands:
Ligand FES 2 x FES
Ligand MOS 1 x MOS
Ligand SAL 1 x SAL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: C2221
Unit cell:
a: 117.831Å b: 165.403Å c: 154.447Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.275

Quick links

Citations

74 review citations

Therapeutic effects of xanthine oxidase inhibitors: renaissance half a century after the discovery of allopurinol.
Pacher et al. (2006)
73 more

41 mentions without citation

The mononuclear molybdenum enzymes.
Hille et al. (2014)
40 more