› 1eyq

X-ray diffraction
1.85Å resolution

Chalcone isomerase and naringenin

Released:
DOI: 10.2210/pdb1eyq/pdb
PDB entry 1eyq coloured by chain, front view.
PDB entry 1eyq coloured by chain, side view.
PDB entry 1eyq coloured by chain, top view.
Source organism: Medicago sativa
Primary publication:
Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase.
Jez JM, Bowman ME, Dixon RA, Noel JP
Nat Struct Biol 7 786-91 (2000)
PMID: 10966651

Function and Biology Details

Reaction catalysed: 
A chalcone = a flavanone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-151152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone--flavanone isomerase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 23.85 KDa
Source organism: Medicago sativa
Expression system: Escherichia coli
UniProt:
  • Canonical: P28012 (Residues: 1-222; Coverage: 100%)
Gene names: CHI-1, CHI1
Sequence domains: Chalcone-flavanone isomerase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand NAR 2 x NAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P6522
Unit cell:
a: 88.624Å b: 88.624Å c: 350.369Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.237 0.262
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Biosynthesis of flavonoids and effects of stress.
Winkel-Shirley B. (2002)
23 more

10 mentions without citation

Structure and function of enzymes involved in the biosynthesis of phenylpropanoids.
Ferrer et al. (2008)
9 more