› 1eww

Solution NMR

SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEGREES CELSIUS

Released:
DOI: 10.2210/pdb1eww/pdb
PDB entry 1eww coloured by chain, ensemble of 20 models, front view.
PDB entry 1eww coloured by chain, ensemble of 20 models, side view.
PDB entry 1eww coloured by chain, ensemble of 20 models, top view.
Source organism: Choristoneura fumiferana
Primary publication:
Beta-helix structure and ice-binding properties of a hyperactive antifreeze protein from an insect.
Graether SP, Kuiper MJ, Gagné SM, Walker VK, Jia Z, Sykes BD, Davies PL
Nature 406 325-8 (2000)
PMID: 10917537

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermal hysteresis protein Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 9.07 KDa
Source organism: Choristoneura fumiferana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GTP0 (Residues: 19-108; Coverage: 100%)
Sequence domains: Choristoneura fumiferana antifreeze protein (CfAFP)
Structure domains: Insect antifreeze protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

29 review citations

Nanoparticles, Proteins, and Nucleic Acids: Biotechnology Meets Materials Science.
Niemeyer CM. (2001)
28 more

6 mentions without citation

Natural beta-sheet proteins use negative design to avoid edge-to-edge aggregation.
Richardson et al. (2002)
5 more