› 1ek9

X-ray diffraction
2.1Å resolution

2.1A X-RAY STRUCTURE OF TOLC: AN INTEGRAL OUTER MEMBRANE PROTEIN AND EFFLUX PUMP COMPONENT FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1ek9/pdb
PDB entry 1ek9 coloured by chain, front view.
PDB entry 1ek9 coloured by chain, side view.
PDB entry 1ek9 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export.
Koronakis V, Sharff A, Koronakis E, Luisi B, Hughes C
Nature 405 914-9 (2000)
PMID: 10879525

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136391 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein TolC Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 428 amino acids
Theoretical weight: 47.24 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P02930 (Residues: 23-450; Coverage: 91%)
Gene names: JW5503, b3035, colE1-i, mtcB, mukA, refI, toc, tolC, weeA
Sequence domains: Outer membrane efflux protein
Structure domains: Outer membrane efflux proteins (OEP)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R3
Unit cell:
a: 265.049Å b: 265.049Å c: 95.959Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.208 0.257
Expression system: Escherichia coli

Quick links

Citations

146 review citations

The bacterial cell envelope.
Silhavy et al. (2010)
145 more

70 mentions without citation

Molecular basis of bacterial outer membrane permeability revisited.
Nikaido H. (2003)
69 more