› 1ejj

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURAL ANALYSIS OF PHOSPHOGLYCERATE MUTASE COCRYSTALLIZED WITH 3-PHOSPHOGLYCERATE

Released:
DOI: 10.2210/pdb1ejj/pdb
PDB entry 1ejj coloured by chain, front view.
PDB entry 1ejj coloured by chain, side view.
PDB entry 1ejj coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structure and mechanism of action of a novel phosphoglycerate mutase from Bacillus stearothermophilus.
Jedrzejas MJ, Chander M, Setlow P, Krishnasamy G
EMBO J 19 1419-31 (2000)
PMID: 10747010

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = 3-phospho-D-glycerate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194920 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,3-bisphosphoglycerate-independent phosphoglycerate mutase Chain: A
Molecule details ›
Chain: A
Length: 511 amino acids
Theoretical weight: 57.02 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Bacillus subtilis
UniProt:
  • Canonical: Q9X519 (Residues: 1-511; Coverage: 100%)
Gene names: gpmI, pgm
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand 3PG 1 x 3PG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 58.486Å b: 206.293Å c: 125.134Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.247
Expression system: Bacillus subtilis

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Citations

5 review citations

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
4 more

8 mentions without citation

Bacterial Sphingomyelinases and Phospholipases as Virulence Factors.
Flores-Díaz et al. (2016)
7 more