› 1ei1

X-ray diffraction
2.3Å resolution

DIMERIZATION OF E. COLI DNA GYRASE B PROVIDES A STRUCTURAL MECHANISM FOR ACTIVATING THE ATPASE CATALYTIC CENTER

Released:
DOI: 10.2210/pdb1ei1/pdb
PDB entry 1ei1 coloured by chain, front view.
PDB entry 1ei1 coloured by chain, side view.
PDB entry 1ei1 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Dimerization of Escherichia coli DNA-gyrase B provides a structural mechanism for activating the ATPase catalytic center.
Brino L, Urzhumtsev A, Mousli M, Bronner C, Mitschler A, Oudet P, Moras D
J Biol Chem 275 9468-75 (2000)
PMID: 10734094

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142497 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA gyrase subunit B Chains: A, B
Molecule details ›
Chains: A, B
Length: 391 amino acids
Theoretical weight: 43.08 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AES6 (Residues: 2-392; Coverage: 49%)
Gene names: JW5625, acrB, b3699, cou, gyrB, himB, hisU, nalC, parA, pcbA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand ANP 2 x ANP
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P21212
Unit cell:
a: 84.7Å b: 137.4Å c: 78.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.166 0.266
Expression system: Escherichia coli

Quick links

Citations

16 review citations

DNA topoisomerases: structure, function, and mechanism.
Champoux JJ. (2001)
15 more

33 mentions without citation

A "Double-Edged" Scaffold: Antitumor Power within the Antibacterial Quinolone.
Bisacchi et al. (2016)
32 more