› 1ehn

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF CHITINASE A MUTANT E315Q COMPLEXED WITH OCTA-N-ACETYLCHITOOCTAOSE (NAG)8.

Released:
DOI: 10.2210/pdb1ehn/pdb
PDB entry 1ehn coloured by chain, front view.
PDB entry 1ehn coloured by chain, side view.
PDB entry 1ehn coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
High resolution structural analyses of mutant chitinase A complexes with substrates provide new insight into the mechanism of catalysis.
Papanikolau Y, Prag G, Tavlas G, Vorgias CE, Oppenheim AB, Petratos K
Biochemistry 40 11338-43 (2001)
PMID: 11560481

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139457 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitinase A Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 58.64 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07254 (Residues: 24-563; Coverage: 100%)
Gene name: chiA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2221
Unit cell:
a: 200.44Å b: 131.617Å c: 59.344Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.217
Expression system: Escherichia coli

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Citations

3 review citations

Glycosidase mechanisms.
Vasella et al. (2002)
2 more

10 mentions without citation

Aromatic residues in the catalytic center of chitinase A from Serratia marcescens affect processivity, enzyme activity, and biomass converting efficiency.
Zakariassen et al. (2009)
9 more