spacer
EBI PDBe PDBeView

PDBe Entry: 1ef0 view

CRYSTAL STRUCTURE OF PI-SCEI MINIPRECURSOR
Summary
Header HYDROLASEsearch
Method X-RAY DIFFRACTION
Experiment Resolution: 2.1 Å, R-factor: 23.1%, Free R-factor: 28.1%, Spacegroup: P 1 21 1
Released 07/06/2000, deposition: 04/02/2000, last revision: 24/02/2009
Authors Poland, B.W.search; Xu, M.-Q.search; Quiocho, F.A.search
Primary citation Structural insights into the protein splicing mechanism of PI-SceI.
J.BIOL.CHEM.search vol:275, pag:16408-16413 (2000) [PubMed ID 10828056 ]search
Keywords endonucleasesearch, protein splicingsearch, mini-precursorsearch, HYDROLASEsearch
EC 3.6.3.14 ExPASy BRENDA search (A B)
Organism Saccharomyces cerevisiae(baker's yeast) 4932search(A B)
UniProt Vacuolar ATP synthase catalytic subunit A (EC 3.6.3.14) (V-ATPase subunit A) (Vacuolar proton pump subunit A) [Contains: Endonuclease PI-SceI (EC 3.1.-.-) (VMA1-derived endonuclease) (VDE) (Sce VMA intein)] P17255search (A B)
Solvent A, B
Polymers
Id Name Type UniProt Residues Observed
A, B PI-SCEI ENDONUCLEASE Protein P17255 (VATA_YEAST)search
 462 91%
Heterogens
Id Name Ligands
B, A ZINC ION ZN search
spacer