› 1ecm

X-ray diffraction
2.2Å resolution

ATOMIC STRUCTURE OF THE BURIED CATALYTIC POCKET OF ESCHERICHIA COLI CHORISMATE MUTASE

Released:
DOI: 10.2210/pdb1ecm/pdb
PDB entry 1ecm coloured by chain, front view.
PDB entry 1ecm coloured by chain, side view.
PDB entry 1ecm coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
ATOMIC-STRUCTURE OF THE BURIED CATALYTIC POCKET OF ESCHERICHIA-COLI CHORISMATE MUTASE.
Lee AY, Karplus PA, Ganem B, Clardy J
J. Am. Chem. Soc. 117 3627-3628 (1995)

Function and Biology Details

Reactions catalysed: 
Prephenate = phenylpyruvate + H(2)O + CO(2)
Chorismate = prephenate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141891 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional chorismate mutase/prephenate dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 109 amino acids
Theoretical weight: 12.55 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P0A9J8 (Residues: 1-109; Coverage: 28%)
Gene names: JW2580, b2599, pheA
Sequence domains: Chorismate mutase type II
Structure domains: Chorismate mutase

Ligands and Environments

1 bound ligand:
Ligand TSA 2 x TSA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3221
Unit cell:
a: 80.83Å b: 80.83Å c: 83.32Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.192 0.231
Expression system: Not provided

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