1ecl Summary

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AMINO TERMINAL 67KDA DOMAIN OF ESCHERICHIA COLI DNA TOPOISOMERASE I (RESIDUES 2-590 OF MATURE PROTEIN) CLONING ARTIFACT ADDS TWO RESIDUES TO THE AMINO-TERMINUS WHICH WERE NOT OBSERVED IN THE EXPERIMENTAL ELECTRON DENSITY (GLY-2, SER-1).

The structure was published by Lima, C.D., Wang, J.C., and Mondragon, A., in 1994 in a paper entitled "Three-dimensional structure of the 67K N-terminal fragment of E. coli DNA topoisomerase I." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.9 Å and deposited in 1995.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of ESCHERICHIA COLI TOPOISOMERASE I. This molecule has the UniProt identifier P06612 (TOP1_ECOLI)search. The sample contained 597 residues which is < 90% of the natural sequence. Out of 597 residues 552 were observed and are deposited in the PDB.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A ESCHERICHIA COLI TOPOISOMERASE I P06612 (1-597) (TOP1_ECOLI)search Escherichia coli K-12search < 90% 597 92%


This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P06612 (1 - 597) ESCHERICHIA COLI TOPOISOMERASE I Escherichia coli

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A Prokaryotic type I DNA topoisomerasesearch Rossmann foldsearch, Topoisomerase I, domain 2search, Topoisomerase I, domain 3search, Topoisomerase I, domain 4search DNA topoisomerasesearch, Topoisomerase DNA binding C4 zinc fingersearch, Toprim domainsearch

Chain ID Biological process (GO) Molecular function (GO)
A (P06612) DNA topological changesearch DNA topoisomerase type I activitysearch DNA topoisomerase activitysearch DNA bindingsearch

Chain InterPro annotation
A DNA topoisomerase, type IAsearch DNA topoisomerase, type IA, domain 2search DNA topoisomerase, type IA, DNA-binding domainsearch DNA topoisomerase I, bacterial-typesearch Toprim domainsearch DNA topoisomerase, type IA, centralsearch DNA topoisomerase, type IA, central region, subdomain 1search DNA topoisomerase, type IA, central region, subdomain 2search DNA topoisomerase, type IA, core domainsearch DNA topoisomerase, type IA, active sitesearch DNA topoisomerase I, type IAsearch