› 1e7l

X-ray diffraction
1.32Å resolution

Endonuclease VII (EndoVII) N62D mutant from phage T4

Released:
DOI: 10.2210/pdb1e7l/pdb
PDB entry 1e7l coloured by chain, front view.
PDB entry 1e7l coloured by chain, side view.
PDB entry 1e7l coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Conformational flexibility in T4 endonuclease VII revealed by crystallography: implications for substrate binding and cleavage.
Raaijmakers H, Törö I, Birkenbihl R, Kemper B, Suck D
J Mol Biol 308 311-23 (2001)
PMID: 11327769

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146548 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Recombination endonuclease VII Chains: A, B
Molecule details ›
Chains: A, B
Length: 157 amino acids
Theoretical weight: 18.17 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13340 (Residues: 1-157; Coverage: 100%)
Gene name: 49
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 14 x SO4
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21
Unit cell:
a: 58.24Å b: 35.83Å c: 91.93Å
α: 90° β: 103.93° γ: 90°
R-values:
R R work R free
0.145 0.146 0.186
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Holliday junction resolvases.
Wyatt et al. (2014)
2 more

4 mentions without citation

Relating destabilizing regions to known functional sites in proteins.
Dessailly et al. (2007)
3 more