› 1e3h

X-ray diffraction
2.6Å resolution

SeMet derivative of Streptomyces antibioticus PNPase/GPSI enzyme

Released:
DOI: 10.2210/pdb1e3h/pdb
PDB entry 1e3h coloured by chain, front view.
PDB entry 1e3h coloured by chain, side view.
PDB entry 1e3h coloured by chain, top view.
Source organism: Streptomyces antibioticus
Primary publication:
A duplicated fold is the structural basis for polynucleotide phosphorylase catalytic activity, processivity, and regulation.
Symmons MF, Jones GH, Luisi BF
Structure 8 1215-26 (2000)
PMID: 11080643

Function and Biology Details

Reaction catalysed: 
RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyribonucleotide nucleotidyltransferase Chain: A
Molecule details ›
Chain: A
Length: 757 amino acids
Theoretical weight: 82.16 KDa
Source organism: Streptomyces antibioticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53597 (Residues: 1-740; Coverage: 100%)
Gene name: pnp
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 9 x SO4
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: R32
Unit cell:
a: 133.61Å b: 133.61Å c: 344.47Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.201 0.226
Expression system: Escherichia coli

Quick links

Citations

42 review citations

The enzymes and control of eukaryotic mRNA turnover.
Parker et al. (2004)
41 more

2 mentions without citation

Crystal structure of Escherichia coli polynucleotide phosphorylase core bound to RNase E, RNA and manganese: implications for catalytic mechanism and RNA degradosome assembly.
Nurmohamed et al. (2009)
1 more