› 1dsz

X-ray diffraction
1.7Å resolution

STRUCTURE OF THE RXR/RAR DNA-BINDING DOMAIN HETERODIMER IN COMPLEX WITH THE RETINOIC ACID RESPONSE ELEMENT DR1

Released:
DOI: 10.2210/pdb1dsz/pdb
PDB entry 1dsz coloured by chain, front view.
PDB entry 1dsz coloured by chain, side view.
PDB entry 1dsz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the RXR-RAR DNA-binding complex on the retinoic acid response element DR1.
Rastinejad F, Wagner T, Zhao Q, Khorasanizadeh S
EMBO J 19 1045-54 (2000)
PMID: 10698945

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113712 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Retinoic acid receptor alpha Chain: A
Molecule details ›
Chain: A
Length: 86 amino acids
Theoretical weight: 10.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10276 (Residues: 82-167; Coverage: 19%)
Gene names: NR1B1, RARA
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
Retinoic acid receptor RXR-alpha Chain: B
Molecule details ›
Chain: B
Length: 85 amino acids
Theoretical weight: 9.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19793 (Residues: 129-212; Coverage: 18%)
Gene names: NR2B1, RXRA
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*CP*AP*GP*GP*TP*CP*AP*AP*AP*GP*GP*TP*CP*AP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.64 KDa
DNA (5'-D(*CP*TP*GP*AP*CP*CP*TP*TP*TP*GP*AP*CP*CP*TP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.54 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 80.66Å b: 33.9Å c: 101.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.267
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Nuclear hormone receptors and gene expression.
Aranda et al. (2001)
22 more

26 mentions without citation

The retinoid X receptors and their ligands.
Dawson et al. (2012)
25 more