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COCRYSTAL STRUCTURE OF RFX-DBD IN COMPLEX WITH ITS COGNATE X-BOX BINDING SITE

X-ray diffraction
1.5Å resolution
PDB entry 1dp7 coloured by chain, front view.
PDB entry 1dp7 coloured by chain, side view.
PDB entry 1dp7 coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct DNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: MHC class II regulatory factor RFX1 and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 11905.66 Å2
Buried surface area: 9341.51 Å2
Dissociation area: 3,334.37 Å2
Dissociation energy (ΔGdiss): 12.59 kcal/mol
Dissociation entropy (TΔSdiss): 32.31 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-113702

Macromolecules

MHC class II regulatory factor RFX1
Chain: P
Length: 76 amino acids
Theoretical weight: 8.83 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P22670 (Residues: 438-513; Coverage: 8%)
Gene name: RFX1
Pfam: RFX DNA-binding domain
InterPro:
CATH: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
SCOP: P4 origin-binding domain-like
MHC class II regulatory factor RFX1 in PDB entry 1dp7, assembly 1, front view.
MHC class II regulatory factor RFX1 in PDB entry 1dp7, assembly 1, side view.
MHC class II regulatory factor RFX1 in PDB entry 1dp7, assembly 1, top view.
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DNA chain D
Name: DNA (5'-D(*CP*GP*(BRU)P*TP*AP*CP*CP*AP*(BRU)P*GP*GP*TP*AP*AP*CP*G)-3')
Representative chains: D
Source organism: Homo sapiens [9606]
Expression system: Not provided
Length: 16 nucleotides
Theoretical weight: 5.03 KDa
DNA (5'-D(*CP*GP*(BRU)P*TP*AP*CP*CP*AP*(BRU)P*GP*GP*TP*AP*AP*CP*G)-3') in PDB entry 1dp7, assembly 1, front view.
DNA (5'-D(*CP*GP*(BRU)P*TP*AP*CP*CP*AP*(BRU)P*GP*GP*TP*AP*AP*CP*G)-3') in PDB entry 1dp7, assembly 1, side view.
DNA (5'-D(*CP*GP*(BRU)P*TP*AP*CP*CP*AP*(BRU)P*GP*GP*TP*AP*AP*CP*G)-3') in PDB entry 1dp7, assembly 1, top view.
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