1dog

X-ray diffraction
2.3Å resolution

REFINED STRUCTURE FOR THE COMPLEX OF 1-DEOXYNOJIRIMYCIN WITH GLUCOAMYLASE FROM (ASPERGILLUS AWAMORI) VAR. X100 TO 2.4 ANGSTROMS RESOLUTION

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159568 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Glucoamylase Chain: A
Molecule details ›
Chain: A
Length: 470 amino acids
Theoretical weight: 50.45 KDa
Source organism: Aspergillus awamori
Expression system: Not provided
UniProt:
  • Canonical: P69327 (Residues: 25-495; Coverage: 76%)
Gene name: GLAA
Sequence domains: Glycosyl hydrolases family 15
Structure domains: Glycosyltransferase

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, NAG, BMA
Carbohydrate polymer : NEW Components: MAN, NAG, BMA
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 116.6Å b: 103.6Å c: 48.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.119 not available not available
Expression system: Not provided