› 1do6

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF SUPEROXIDE REDUCTASE IN THE OXIDIZED STATE AT 2.0 ANGSTROM RESOLUTION

Released:
DOI: 10.2210/pdb1do6/pdb
PDB entry 1do6 coloured by chain, front view.
PDB entry 1do6 coloured by chain, side view.
PDB entry 1do6 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Structures of the superoxide reductase from Pyrococcus furiosus in the oxidized and reduced states.
Yeh AP, Hu Y, Jenney FE, Adams MW, Rees DC
Biochemistry 39 2499-508 (2000)
PMID: 10704199

Function and Biology Details

Reaction catalysed: 
Reduced rubredoxin + superoxide + 2 H(+) = oxidized rubredoxin + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160627 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 14.34 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P82385 (Residues: 1-124; Coverage: 100%)
Gene names: PF1281, sorA
Sequence domains: Desulfoferrodoxin
Structure domains: SOR catalytic domain

Ligands and Environments

1 bound ligand:
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P21212
Unit cell:
a: 50.31Å b: 94.02Å c: 52.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.189
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Iron-sulfur protein folds, iron-sulfur chemistry, and evolution.
Meyer J. (2008)
8 more

5 mentions without citation

Superoxide dismutases and superoxide reductases.
Sheng et al. (2014)
4 more