› 1do0

X-ray diffraction
3Å resolution

ORTHORHOMBIC CRYSTAL FORM OF HEAT SHOCK LOCUS U (HSLU) FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1do0/pdb
PDB entry 1do0 coloured by chain, front view.
PDB entry 1do0 coloured by chain, side view.
PDB entry 1do0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structures of HsIU and the ATP-dependent protease HsIU-HsIV.
Bochtler M, Hartmann C, Song HK, Bourenkov GP, Bartunik HD, Huber R
Nature 403 800-5 (2000)
PMID: 10693812

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141352 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent protease ATPase subunit HslU Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 442 amino acids
Theoretical weight: 49.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6H5 (Residues: 2-443; Coverage: 100%)
Gene names: JW3902, b3931, hslU, htpI
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 4 x ATP
Ligand MG 2 x MG
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P21212
Unit cell:
a: 208.15Å b: 167.7Å c: 108.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.294 0.294 0.342
Expression system: Escherichia coli BL21(DE3)

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Citations

67 review citations

Recognition and processing of ubiquitin-protein conjugates by the proteasome.
Finley D. (2009)
66 more

20 mentions without citation

Fundamental Characteristics of AAA+ Protein Family Structure and Function.
Miller et al. (2016)
19 more