› 1dnk

X-ray diffraction
2.3Å resolution

THE X-RAY STRUCTURE OF THE DNASE I-D(GGTATACC)2 COMPLEX AT 2.3 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1dnk/pdb
PDB entry 1dnk coloured by chain, front view.
PDB entry 1dnk coloured by chain, side view.
PDB entry 1dnk coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
X-ray structure of the DNase I-d(GGTATACC)2 complex at 2.3 A resolution.
Weston SA, Lahm A, Suck D
J Mol Biol 226 1237-56 (1992)
PMID: 1518054

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113697 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (4 distinct):
Deoxyribonuclease-1 Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 29.09 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00639 (Residues: 23-282; Coverage: 100%)
Gene names: DNASE1, DNL1
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase
DNA (5'-D(*GP*GP*TP*AP*TP*AP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 7 nucleotides
Theoretical weight: 2.14 KDa
Source organism: Bos taurus
Expression system: Not provided
DNA (5'-D(*GP*GP*TP*AP*TP*AP*CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 8 nucleotides
Theoretical weight: 2.43 KDa
Source organism: Bos taurus
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 51.1Å b: 108.4Å c: 62.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 not available not available
Expression system: Not provided

Quick links

Citations

13 review citations

Sphingomyelinases: enzymology and membrane activity.
Goñi et al. (2002)
12 more

15 mentions without citation

iGNM: a database of protein functional motions based on Gaussian Network Model.
Yang et al. (2005)
14 more