› 1dlv

X-ray diffraction
2.29Å resolution

BIOSYNTHETIC THIOLASE FROM ZOOGLOEA RAMIGERA IN COMPLEX WITH COA

Released:
DOI: 10.2210/pdb1dlv/pdb
PDB entry 1dlv coloured by chain, front view.
PDB entry 1dlv coloured by chain, side view.
PDB entry 1dlv coloured by chain, top view.
Source organism: Zoogloea ramigera
Primary publication:
Crystallographic analysis of the reaction pathway of Zoogloea ramigera biosynthetic thiolase.
Modis Y, Wierenga RK
J Mol Biol 297 1171-82 (2000)
PMID: 10764581

Function and Biology Details

Reaction catalysed: 
(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139369 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-CoA acetyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 389 amino acids
Theoretical weight: 40.26 KDa
Source organism: Zoogloea ramigera
Expression system: Escherichia coli
UniProt:
  • Canonical: P07097 (Residues: 5-11, 12-392; Coverage: 99%)
Gene names: phaA, phbA
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments


Cofactor: Ligand COA 4 x COA
1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P21
Unit cell:
a: 84.818Å b: 79.302Å c: 149.979Å
α: 90° β: 93.49° γ: 90°
R-values:
R R work R free
0.242 0.242 0.293
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Enzymes of the mevalonate pathway of isoprenoid biosynthesis.
Miziorko HM. (2011)
7 more

3 mentions without citation

Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum.
Kim et al. (2015)
2 more