› 1dku

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURES OF BACILLUS SUBTILIS PHOSPHORIBOSYLPYROPHOSPHATE SYNTHETASE: MOLECULAR BASIS OF ALLOSTERIC INHIBITION AND ACTIVATION.

Released:
DOI: 10.2210/pdb1dku/pdb
PDB entry 1dku coloured by chain, front view.
PDB entry 1dku coloured by chain, side view.
PDB entry 1dku coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural basis for the function of Bacillus subtilis phosphoribosyl-pyrophosphate synthetase.
Eriksen TA, Kadziola A, Bentsen AK, Harlow KW, Larsen S
Nat Struct Biol 7 303-8 (2000)
PMID: 10742175

Function and Biology Details

Reaction catalysed: 
ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-146870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribose-phosphate pyrophosphokinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 34.91 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P14193 (Residues: 1-317; Coverage: 100%)
Gene names: BSU00510, prs
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand AP2 2 x AP2
Ligand ABM 2 x ABM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P63
Unit cell:
a: 115.65Å b: 115.65Å c: 106.41Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.198 0.241
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Nucleotide metabolism and its control in lactic acid bacteria.
Kilstrup et al. (2005)
5 more

9 mentions without citation

Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.
Hove-Jensen et al. (2017)
8 more