› 1dki

X-ray diffraction
1.6Å resolution

CRYSTAL STRUCTURE OF THE ZYMOGEN FORM OF STREPTOCOCCAL PYROGENIC EXOTOXIN B ACTIVE SITE (C47S) MUTANT

Released:
DOI: 10.2210/pdb1dki/pdb
PDB entry 1dki coloured by chain, front view.
PDB entry 1dki coloured by chain, side view.
PDB entry 1dki coloured by chain, top view.
Source organism: Streptococcus pyogenes
Primary publication:
Crystal structure of the zymogen form of the group A Streptococcus virulence factor SpeB: an integrin-binding cysteine protease.
Kagawa TF, Cooney JC, Baker HM, McSweeney S, Liu M, Gubba S, Musser JM, Baker EN
Proc Natl Acad Sci U S A 97 2235-40 (2000)
PMID: 10681429

Function and Biology Details

Reaction catalysed: 
Preferential cleavage with hydrophobic residues at P2, P1 and P1'.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142885 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptopain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 371 amino acids
Theoretical weight: 40.32 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C0J0 (Residues: 28-398; Coverage: 100%)
Gene name: speB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 46.664Å b: 116.556Å c: 144.726Å
α: 90° β: 94.17° γ: 90°
R-values:
R R work R free
0.219 0.218 0.245
Expression system: Escherichia coli

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Citations

12 review citations

Staphylococcal and streptococcal superantigen exotoxins.
Spaulding et al. (2013)
11 more

7 mentions without citation

Mechanisms Applied by Protein Inhibitors to Inhibit Cysteine Proteases.
TuĊĦar et al. (2021)
6 more