› 1dkg

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF THE NUCLEOTIDE EXCHANGE FACTOR GRPE BOUND TO THE ATPASE DOMAIN OF THE MOLECULAR CHAPERONE DNAK

Released:
DOI: 10.2210/pdb1dkg/pdb
PDB entry 1dkg coloured by chain, front view.
PDB entry 1dkg coloured by chain, side view.
PDB entry 1dkg coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the nucleotide exchange factor GrpE bound to the ATPase domain of the molecular chaperone DnaK.
Harrison CJ, Hayer-Hartl M, Di Liberto M, Hartl F, Kuriyan J
Science 276 431-5 (1997)
PMID: 9103205

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140620 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein GrpE Chains: A, B
Molecule details ›
Chains: A, B
Length: 197 amino acids
Theoretical weight: 21.88 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P09372 (Residues: 1-197; Coverage: 100%)
Gene names: JW2594, b2614, grpE
Sequence domains: GrpE
Structure domains:
Chaperone protein DnaK Chain: D
Molecule details ›
Chain: D
Length: 383 amino acids
Theoretical weight: 41.57 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6Y8 (Residues: 1-383; Coverage: 60%)
Gene names: JW0013, b0014, dnaK, groP, grpF, seg
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P41
Unit cell:
a: 149.43Å b: 149.43Å c: 49.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.317
Expression system: Escherichia coli

Quick links

Citations

54 review citations

The Hsp70 and Hsp60 chaperone machines.
Bukau et al. (1998)
53 more

54 mentions without citation

Hsp70 chaperones: cellular functions and molecular mechanism.
Mayer et al. (2005)
53 more