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EBI PDBe PDBeView

PDBe Entry: 1dj9 view

CRYSTAL STRUCTURE OF 8-AMINO-7-OXONANOATE SYNTHASE (OR 7-KETO-8AMINIPELARGONATE OR KAPA SYNTHASE) COMPLEXED WITH PLP AND THE PRODUCT 8(S)-AMINO-7-OXONANONOATE (OR KAPA). THE ENZYME OF BIOTIN BIOSYNTHETIC PATHWAY.
Summary
Header TRANSFERASEsearch
Method X-RAY DIFFRACTION
Experiment Resolution: 2.0 Å, R-factor: 21.2%, Free R-factor: 27.8%, Spacegroup: P 31 1 2
Released 06/12/2000, deposition: 02/12/1999, last revision: 24/02/2009
Authors Webster, S.P.search; Alexeev, D.search; Campopiano, D.J.search; Watt, R.M.search; Alexeeva, M.search; Sawyer, L.search; Baxter, R.L.search
Primary citation Mechanism of 8-amino-7-oxononanoate synthase: spectroscopic, kinetic, and crystallographic studies.
BIOCHEMISTRYsearch vol:39, pag:516-528 (2000) [PubMed ID 10642176 ]search
Keywords 8-AMINO-7-OXONONANOATE SYNTHASEsearch, BIOTINsearch, PYRIDOXAL-5'-PHOSPHATEsearch, TRANSFERASEsearch
EC 2.3.1.47 ExPASy BRENDA search (A)
Organism Escherichia coli 562search(A)
UniProt 8-amino-7-oxononanoate synthase (EC 2.3.1.47) (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synthetase) (7-KAP synthetase) (L-alanine--pimelyl-CoA ligase) P12998search (A)
Solvent A
Related entries 1bs0
Polymers
Id Name Type UniProt Residues Observed
A 8-AMINO-7-OXONONANOATE SYNTHASE Protein P12998 (BIOF_ECOLI)search
 384 99%
Heterogens
Id Name Ligands
A SULFATE ION SO4 search
A MAGNESIUM ION MG search
A N-[7-KETO-8-AMINOPELARGONIC ACID]-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YL-METHANE] KAM search
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