1diz Summary

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CRYSTAL STRUCTURE OF E. COLI 3-METHYLADENINE DNA GLYCOSYLASE (ALKA) COMPLEXED WITH DNA

The structure was published by Hollis, T., Ichikawa, Y., and Ellenberger, T., in 2000 in a paper entitled "DNA bending and a flip-out mechanism for base excision by the helix-hairpin-helix DNA glycosylase, Escherichia coli AlkA." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.5 Å and deposited in 1999.

The experimental data on which the structure is based was not deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely DNA (5'-D(*GP*AP*CP*AP*TP*GP*AP*(NRI)P*TP*GP*CP*CP*T)-3'), DNA (5'-D(*GP*GP*CP*AP*AP*TP*CP*AP*TP*GP*TP*CP*A)-3'), and 3-METHYLADENINE DNA GLYCOSYLASE II.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A 3-METHYLADENINE DNA GLYCOSYLASE II P04395 (1-282) (3MG2_ECOLI)search Escherichia coli K-12search 100% 282 100%
B 3-METHYLADENINE DNA GLYCOSYLASE II P04395 (1-282) (3MG2_ECOLI)search Escherichia coli K-12search 100% 282 100%


This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P04395 (1 - 282) 3-METHYLADENINE DNA GLYCOSYLASE II Escherichia coli

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B (P04395) DNA repair glycosylase, 2 C-terminal domainssearch, DNA repair glycosylase, N-terminal domainsearch DNA-3-methyladenine glycosylase iisearch, Hypothetical protein; domain 2search, Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)search PF00730: HhH-GPD superfamily base excision DNA repair proteinsearch, PF06029: AlkA N-terminal domainsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A, B (P04395) DNA repairsearch cellular response to DNA damage stimulussearch base-excision repairsearch DNA dealkylation involved in DNA repairsearch DNA-3-methyladenine glycosylase activitysearch DNA-3-methylguanine glycosylase activitysearch DNA-7-methylguanine glycosylase activitysearch alkylbase DNA N-glycosylase activitysearch hydrolase activitysearch DNA-7-methyladenine glycosylase activitysearch cytoplasmsearch

Chain InterPro annotation
A, B Alkylbase DNA glycosidase, conserved sitesearch HhH-GPD domainsearch DNA-3-methyladenine glycosylase AlkA, N-terminalsearch DNA glycosylasesearch Helix-turn-helix, base-excision DNA repair, C-terminalsearch