1d8d Summary

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CO-CRYSTAL STRUCTURE OF RAT PROTEIN FARNESYLTRANSFERASE COMPLEXED WITH A K-RAS4B PEPTIDE SUBSTRATE AND FPP ANALOG AT 2.0A RESOLUTION

The structure was published by Long, S.B., Casey, P.J., and Beese, L.S., in 2000 in a paper entitled "The basis for K-Ras4B binding specificity to protein farnesyltransferase revealed by 2 A resolution ternary complex structures." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.0 Å and deposited in 1999.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely farnesyltransferase (alpha subunit), farnesyltransferase (beta subunit), and K-RAS4B PEPTIDE SUBSTRATE.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterotrimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A farnesyltransferase (alpha subunit) Q04631 (1-377) (FNTA_RAT)search Rattus norvegicussearch 100% 377 85%
B farnesyltransferase (beta subunit) Q02293 (1-437) (FNTB_RAT)search Rattus norvegicussearch 91% 437 93%
P K-RAS4B PEPTIDE SUBSTRATE P01116 (178-188) (RASK_HUMAN)search Homo sapienssearch < 90% 11 100%


This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
Q04631 (1 - 377) farnesyltransferase (alpha subunit) Rattus norvegicus
Q02293 (1 - 437) farnesyltransferase (beta subunit) Rattus norvegicus
P01116 (178 - 188) K-RAS4B PEPTIDE SUBSTRATE

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A (Q04631) Protein prenylyltransferasesearch Protein prenylyltransferasesearch PF01239: Protein prenyltransferase alpha subunit repeatsearch
B (Q02293) Protein prenyltransferasessearch Glycosyltransferasesearch PF00432: Prenyltransferase and squalene oxidase repeatsearch, PF13249: Prenyltransferase-likesearch
P

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A (Q04631) protein geranylgeranyltransferase activitysearch transferase activitysearch protein bindingsearch isoprenoid bindingsearch CAAX-protein geranylgeranyltransferase activitysearch prenyltransferase activitysearch zinc ion bindingsearch protein prenyltransferase activitysearch protein farnesyltransferase activitysearch peptide bindingsearch drug bindingsearch negative regulation of nitric-oxide synthase biosynthetic processsearch response to cytokinesearch positive regulation of cell proliferationsearch positive regulation of cell cyclesearch protein prenylationsearch positive regulation of nitric-oxide synthase biosynthetic processsearch response to organic cyclic compoundsearch response to inorganic substancesearch protein farnesylationsearch negative regulation of apoptotic processsearch protein geranylgeranylationsearch protein farnesyltransferase complexsearch cytoplasmsearch CAAX-protein geranylgeranyltransferase complexsearch
B (Q02293) protein bindingsearch drug bindingsearch farnesyltranstransferase activitysearch protein farnesyltransferase activitysearch isoprenoid bindingsearch prenyltransferase activitysearch catalytic activitysearch zinc ion bindingsearch metal ion bindingsearch peptide bindingsearch transferase activitysearch positive regulation of cell cyclesearch response to cytokinesearch response to organic cyclic compoundsearch positive regulation of fibroblast proliferationsearch protein farnesylationsearch negative regulation of cell proliferationsearch positive regulation of nitric-oxide synthase biosynthetic processsearch regulation of cell proliferationsearch wound healingsearch positive regulation of cell proliferationsearch response to inorganic substancesearch microtubule associated complexsearch protein farnesyltransferase complexsearch protein complexsearch

Chain InterPro annotation
A Protein prenyltransferase, alpha subunitsearch
B Prenyltransferase/squalene oxidasesearch Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidsearch Protein farnesyltransferase subunit betasearch
P