› 1d4z

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF CHEY-95IV, A HYPERACTIVE CHEY MUTANT

Released:
DOI: 10.2210/pdb1d4z/pdb
PDB entry 1d4z coloured by chain, front view.
PDB entry 1d4z coloured by chain, side view.
PDB entry 1d4z coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Correlated switch binding and signaling in bacterial chemotaxis.
Schuster M, Zhao R, Bourret RB, Collins EJ
J Biol Chem 275 19752-8 (2000)
PMID: 10748173

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chemotaxis protein CheY Chain: A
Molecule details ›
Chain: A
Length: 128 amino acids
Theoretical weight: 13.97 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE67 (Residues: 2-129; Coverage: 99%)
Gene names: JW1871, b1882, cheY
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 45.023Å b: 46.849Å c: 53.583Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.227
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Diversity in chemotaxis mechanisms among the bacteria and archaea.
Szurmant et al. (2004)
1 more

2 mentions without citation

Characterization of the fast dynamics of protein amino acid side chains using NMR relaxation in solution.
Igumenova et al. (2006)
1 more