› 1d3v

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE OF THE BINUCLEAR MANGANESE METALLOENZYME ARGINASE COMPLEXED WITH 2(S)-AMINO-6-BORONOHEXANOIC ACID, AN L-ARGININE ANALOG

Released:
DOI: 10.2210/pdb1d3v/pdb
PDB entry 1d3v coloured by chain, front view.
PDB entry 1d3v coloured by chain, side view.
PDB entry 1d3v coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Arginase-boronic acid complex highlights a physiological role in erectile function.
Cox JD, Kim NN, Traish AM, Christianson DW
Nat Struct Biol 6 1043-7 (1999)
PMID: 10542097

Function and Biology Details

Reaction catalysed: 
L-arginine + H(2)O = L-ornithine + urea
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139779 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginase-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 35.02 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07824 (Residues: 1-323; Coverage: 100%)
Gene name: Arg1
Sequence domains: Arginase family
Structure domains: Ureohydrolase domain

Ligands and Environments

2 bound ligands:
Ligand MN 4 x MN
Ligand ABH 2 x ABH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P3
Unit cell:
a: 91.3Å b: 91.3Å c: 69.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.157 0.179
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Regulation of enzymes of the urea cycle and arginine metabolism.
Morris SM. (2002)
21 more

6 mentions without citation

Crystal structure of human arginase I at 1.29-A resolution and exploration of inhibition in the immune response.
Di Costanzo et al. (2005)
5 more