› 1d2a

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF A YEAST LOW MOLECULAR WEIGHT PROTEIN TYROSINE PHOSPHATASE (LTP1) COMPLEXED WITH THE ACTIVATOR ADENINE

Released:
DOI: 10.2210/pdb1d2a/pdb
PDB entry 1d2a coloured by chain, front view.
PDB entry 1d2a coloured by chain, side view.
PDB entry 1d2a coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural and mechanistic basis for the activation of a low-molecular weight protein tyrosine phosphatase by adenine.
Wang S, Stauffacher CV, Van Etten RL
Biochemistry 39 1234-42 (2000)
PMID: 10684601

Function and Biology Details

Reactions catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154100 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Low molecular weight phosphotyrosine protein phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 160 amino acids
Theoretical weight: 18.54 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P40347 (Residues: 2-161; Coverage: 99%)
Gene names: LTP1, YP9499.28C, YPR073C
Sequence domains: Low molecular weight phosphotyrosine protein phosphatase
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand PO4 2 x PO4
Ligand CL 1 x CL
Ligand ADE 1 x ADE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 51.492Å b: 63.272Å c: 97.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.243
Expression system: Escherichia coli

Quick links

Citations

11 citation in other articles

Ssu72 is a phosphatase essential for transcription termination of snoRNAs and specific mRNAs in yeast.
Ganem et al. (2003)
10 more

5 mentions without citation

The Bombyx mori karyotype and the assignment of linkage groups.
Yoshido et al. (2005)
4 more