› 1cz0

X-ray diffraction
2.1Å resolution

INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION

Released:
DOI: 10.2210/pdb1cz0/pdb
PDB entry 1cz0 coloured by chain, front view.
PDB entry 1cz0 coloured by chain, side view.
PDB entry 1cz0 coloured by chain, top view.
Source organism: Physarum polycephalum
Primary publication:
A novel endonuclease mechanism directly visualized for I-PpoI.
Galburt EA, Chevalier B, Tang W, Jurica MS, Flick KE, Monnat RJ, Stoddard BL
Nat Struct Biol 6 1096-9 (1999)
PMID: 10581547

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113567 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Intron-encoded endonuclease I-PpoI Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 17.68 KDa
Source organism: Physarum polycephalum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94702 (Residues: 2-163; Coverage: 99%)
Sequence domains: Zinc-binding loop region of homing endonuclease
Structure domains: Homing Intron 3 (I-ppo) Encoded Endonuclease; Chain A
DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*AP*GP*AP*GP*AP*GP*TP*CP*A)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 21 nucleotides
Theoretical weight: 6.44 KDa
Source organism: Physarum polycephalum
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3121
Unit cell:
a: 113.866Å b: 113.866Å c: 89.04Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.191 0.216
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Structure and function of type II restriction endonucleases.
Pingoud et al. (2001)
9 more