› 1cuk

X-ray diffraction
1.9Å resolution

ESCHERICHIA COLI RUVA PROTEIN AT PH 4.9 AND ROOM TEMPERATURE

Released:
DOI: 10.2210/pdb1cuk/pdb
PDB entry 1cuk coloured by chain, front view.
PDB entry 1cuk coloured by chain, side view.
PDB entry 1cuk coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of DNA recombination protein RuvA and a model for its binding to the Holliday junction.
Rafferty JB, Sedelnikova SE, Hargreaves D, Artymiuk PJ, Baker PJ, Sharples GJ, Mahdi AA, Lloyd RG, Rice DW
Science 274 415-21 (1996)
PMID: 8832889

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141650 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Holliday junction branch migration complex subunit RuvA Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 22.2 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A809 (Residues: 1-203; Coverage: 100%)
Gene names: JW1850, b1861, ruvA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P4
Unit cell:
a: 83.7Å b: 83.7Å c: 33.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 not available
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Structure and mechanism of helicases and nucleic acid translocases.
Singleton et al. (2007)
27 more

9 mentions without citation

The InterPro protein families and domains database: 20 years on.
Blum et al. (2021)
8 more