spacer STRUCTURE OF THE MAMMALIAN CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE AND AN INHIBITOR PEPTIDE DISPLAYS AN OPEN CONFORMATION
Primary citation
Title Structure of the mammalian catalytic subunit of cAMP-dependent protein kinase and an inhibitor peptide displays an open conformation.
Authors Karlsson, R.search; Zheng, J.search; Xuong, N.search; Taylor, S.S.search; Sowadski, J.M.search
Journal ACTA CRYSTALLOGR.,SECT.Dsearch vol:49, pag:381-388 (1993), Identifiers: PubMed ID (15299513)search DOI (10.1107/S0907444993002306)
Abstract The crystal structure of a binary complex of the porcine heart catalytic (C) subunit of cAMP-dependent protein kinase (space group P4(1)32; a = 171.5 A) complexed with a di-iodinated peptide inhibitor, PKI(5-24), has been solved and refined to 2.9 A resolution with an overall R of 21.1%. The r.m.s. deviations from ideal bond lengths and angles are 0.022 A and 4.3 degrees. A single isotropic B of 17 A(2) was used for all atoms. The structure solution was carried out initially by molecular replacement of electron density followed by refinement against atomic coordinates from orthorhombic crystals of a binary complex of the mouse recombinant enzyme previously described [Knighton, Zheng, Ten Eyck, Ashford, Xuong, Taylor & Sowadski (1991). Science, 253, 407-414]. The most striking difference between the two crystal structures is a large displacement of the small lobe of the enzyme. In the cubic crystal, the beta-sheet of the small lobe is rotated by 15 degrees and translated by 1.9 A with respect to the orthorhombic crystal. Possible explanations for why this binary complex crystallized in an open conformation in contrast to a similar binary complex of the recombinant enzyme are discussed. This study demonstrates that considerable information about parts of a crystal structure can be obtained without a complete crystal structure analysis. Specifically, the six rigid-group parameters of a poly alanine model of the beta-structure were obtained satisfactorily from a crystal structure by refinement of difference Fourier coefficients based on an approximate partial structure model.
MeSH terms
Secondary citations
Title Crystal-Structure of the Catalytic Subunit of Camp-Dependent Protein-Kinase Complexed with a Mgatp and Peptide Inhibitor
Authors Zheng, J.search; Knighton, D.R.search; Xuong, N.-H.search; Eyck, L.F.T.search; Karlsson, R.search; Taylor, S.S.search; Sowadski, J.M.search
Journal BIOCHEMISTRYsearch vol:32, pag:2154 (1993)
Title 2.0 Angstroms Refined Crystal Structure of the Catalytic Subunit of Camp-Dependent Protein Kinase Complexed with a Peptide Inhibitor and Detergent
Authors Knighton, D.R.search; Bell, S.M.search; Zheng, J.search; Eyck, L.F.T.search; Xuong, N.-H.search; Taylor, S.S.search; Sowadski, J.M.search
Journal ACTA CRYSTALLOGR.,SECT.Dsearch vol:49, pag:357 (1993)
Title 2.2 Angstroms Refined Crystal-Structure of the Catalytic Subunit of Camp-Dependent Protein-Kinase Complexed with Mnatp and a Peptide Inhibitor
Authors Zheng, J.H.search; Trafny, E.A.search; Knighton, D.R.search; Xuong, N.-H.search; Taylor, S.S.search; Eyck, L.F.T.search; Sowadski, J.M.search
Journal ACTA CRYSTALLOGR.,SECT.Dsearch vol:49, pag:362 (1993)
Title Crystal Structure of the Catalytic Subunit of Cyclic Adenosine Monophosphate-Dependent Protein Kinase
Authors Knighton, D.R.search; Zheng, J.search; Eyck, L.F.T.search; Ashford, V.A.search; Xuong, N.-H.search; Taylor, S.S.search; Sowadski, J.M.search
Journal SCIENCEsearch vol:253, pag:407 (1991)
Title Structure of a Peptide Inhibitor Bound to the Catalytic Subunit of Cyclic Adenosine Monophosphate-Dependent Protein Kinase
Authors Knighton, D.R.search; Zheng, J.search; Eyck, L.F.T.search; Xuong, N.-H.search; Taylor, S.S.search; Sowadski, J.M.search
Journal SCIENCEsearch vol:253, pag:414 (1991)
Title Expression of the Catalytic Subunit of Camp-Dependent Protein Kinase in Escherichia Coli
Authors Slice, L.W.search; Taylor, S.S.search
Journal J.BIOL.CHEM.search vol:264, pag:20940 (1989)
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