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Determination of the solution structure of a synthetic two-site calcium-binding homodimeric protein domain by NMR spectroscopy.

Shaw GS, Hodges RS, Sykes BD
Biochemistry 31 9572-80 (1992)
Cited: 21 times
EuropePMC logo PMID: 1390738

Abstract

The solution structure of a 34-residue synthetic calcium-binding peptide from site III of chicken troponin-C has been determined by 1H NMR spectroscopy. In solution and in the presence of calcium this peptide forms a symmetric two-site homodimeric calcium-binding domain (Shaw et al., 1990). The solution structure of this dimer was determined from the measurement of 470 NOEs from a 75-ms NOESY data set. For the dimer structure determination, the constraint list included 868 distance restraints, 44 phi angles, and 24 chi 1 and 2 chi 2 angles. Seven structures were calculated by restrained molecular dynamics using a procedure in which intramonomer distances were used first and then all distances, intra- and intermonomer, were input during further dynamics. The structures exhibited a fold very similar to the C-terminal domain of troponin-C comprised of a pair of helix-loop-helix calcium-binding sites. The rms deviation of these structures for backbone atoms between residues 97-122 and 97'-122' for the dimer was 0.82 A. The dimer structure was also calculated to be more symmetric than sites III and IV in troponin-C.

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Reviews citing this publication (1)

  1. Protein dynamics. Jardetzky O, Lefèvre JF. FEBS Lett 338 246-250 (1994)

Articles citing this publication (19)

  1. Backbone and methyl dynamics of the regulatory domain of troponin C: anisotropic rotational diffusion and contribution of conformational entropy to calcium affinity. Gagné SM, Tsuda S, Spyracopoulos L, Kay LE, Sykes BD. J Mol Biol 278 667-686 (1998)
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  13. Relative stabilities of synthetic peptide homo- and heterodimeric troponin-C domains. Shaw GS, Hodges RS, Kay CM, Sykes BD. Protein Sci 3 1010-1019 (1994)
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  15. Characterization of a helix-loop-helix (EF hand) motif of silver hake parvalbumin isoform B. Revett SP, King G, Shabanowitz J, Hunt DF, Hartman KL, Laue TM, Nelson DJ. Protein Sci 6 2397-2408 (1997)
  16. Human S100b protein: formation of a tetramer from synthetic calcium-binding site peptides. Donaldson C, Barber KR, Kay CM, Shaw GS. Protein Sci 4 765-772 (1995)
  17. Effects of high pressure and temperature on the wild-type and F29W mutant forms of the N-domain of avian troponin C. Yu A, Ballard L, Smillie L, Pearlstone J, Foguel D, Silva J, Jonas A, Jonas J. Biochim Biophys Acta 1431 53-63 (1999)
  18. Investigating site-specific effects of the -X glutamate in a parvalbumin CD site model peptide. Franchini PL, Reid RE. Arch Biochem Biophys 372 80-88 (1999)
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