1cs0 Summary

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Crystal structure of carbamoyl phosphate synthetase complexed at CYS269 in the small subunit with the tetrahedral mimic l-glutamate gamma-semialdehyde

The structure was published by Thoden, J.B., Huang, X., Raushel, F.M., and Holden, H.M., in 1999 in a paper entitled "The small subunit of carbamoyl phosphate synthetase: snapshots along the reaction pathway." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.0 Å and deposited in 1999.

The experimental data on which the structure is based was not deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely CARBAMOYL PHOSPHATE SYNTHETASE: LARGE SUBUNIT and CARBAMOYL PHOSPHATE SYNTHETASE: SMALL SUBUNIT.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A CARBAMOYL PHOSPHATE SYNTHETASE: LARGE SUBUNIT P00968 (1-1073) (CARB_ECOLI)search Escherichia coli K-12search 100% 1073 98%
C CARBAMOYL PHOSPHATE SYNTHETASE: LARGE SUBUNIT P00968 (1-1073) (CARB_ECOLI)search Escherichia coli K-12search 100% 1073 98%
E CARBAMOYL PHOSPHATE SYNTHETASE: LARGE SUBUNIT P00968 (1-1073) (CARB_ECOLI)search Escherichia coli K-12search 100% 1073 98%
G CARBAMOYL PHOSPHATE SYNTHETASE: LARGE SUBUNIT P00968 (1-1073) (CARB_ECOLI)search Escherichia coli K-12search 100% 1073 98%
B CARBAMOYL PHOSPHATE SYNTHETASE: SMALL SUBUNIT P0A6F1 (1-382) (CARA_ECOLI)search Escherichia coli K-12search 99% 382 99%
D CARBAMOYL PHOSPHATE SYNTHETASE: SMALL SUBUNIT P0A6F1 (1-382) (CARA_ECOLI)search Escherichia coli K-12search 99% 382 99%
F CARBAMOYL PHOSPHATE SYNTHETASE: SMALL SUBUNIT P0A6F1 (1-382) (CARA_ECOLI)search Escherichia coli K-12search 99% 382 99%
H CARBAMOYL PHOSPHATE SYNTHETASE: SMALL SUBUNIT P0A6F1 (1-382) (CARA_ECOLI)search Escherichia coli K-12search 99% 382 99%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P00968 (1 - 1073) CARBAMOYL PHOSPHATE SYNTHETASE: LARGE SUBUNIT Escherichia coli
P0A6F1 (1 - 382) CARBAMOYL PHOSPHATE SYNTHETASE: SMALL SUBUNIT Escherichia coli

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, C, E, G (P00968) Carbamoyl phosphate synthetase, large subunit connection domainsearch, Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domainsearch, BC N-terminal domain-likesearch, BC ATP-binding domain-likesearch Rossmann foldsearch, ATP-grasp fold, B domainsearch, ATP-grasp fold, A domainsearch, Carbamoyl Phosphate Synthetase; Chain A, domain 4search PF00289: Carbamoyl-phosphate synthase L chain, N-terminal domainsearch, PF02142: MGS-like domainsearch, PF02786: Carbamoyl-phosphate synthase L chain, ATP binding domainsearch, PF02787: Carbamoyl-phosphate synthetase large chain, oligomerisation domainsearch
B, D, F, H (P0A6F1) Carbamoyl phosphate synthetase, small subunit N-terminal domainsearch, Class I glutamine amidotransferases (GAT)search Carbamoyl phosphate synthetase, small subunit N-terminal domainsearch, Rossmann foldsearch PF00117: Glutamine amidotransferase class-Isearch, PF00988: Carbamoyl-phosphate synthase small chain, CPSase domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, C, E, G (P00968) ATP bindingsearch catalytic activitysearch metal ion bindingsearch protein bindingsearch nucleotide bindingsearch carbamoyl-phosphate synthase (glutamine-hydrolyzing) activitysearch ligase activitysearch amino acid bindingsearch carbamoyl-phosphate synthase (ammonia) activitysearch nitrogen compound metabolic processsearch metabolic processsearch cellular amino acid biosynthetic processsearch arginine biosynthetic processsearch 'de novo' UMP biosynthetic processsearch urea cyclesearch pyrimidine nucleobase biosynthetic processsearch pyrimidine nucleotide biosynthetic processsearch biofilm formationsearch carbamoyl-phosphate synthase complexsearch
B, D, F, H (P0A6F1) carbamoyl-phosphate synthase (glutamine-hydrolyzing) activitysearch protein bindingsearch ligase activitysearch nucleotide bindingsearch ATP bindingsearch glutamine catabolic processsearch carbamoyl phosphate biosynthetic processsearch pyrimidine nucleotide biosynthetic processsearch arginine biosynthetic processsearch glutamine metabolic processsearch urea cyclesearch 'de novo' pyrimidine nucleobase biosynthetic processsearch cellular amino acid biosynthetic processsearch 'de novo' UMP biosynthetic processsearch cytosolsearch cytoplasmsearch carbamoyl-phosphate synthase complexsearch

Chain InterPro annotation
A, C, E, G Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domainsearch Carbamoyl-phosphate synthetase, large subunit oligomerisation domainsearch Carbamoyl-phosphate synthase, large subunit, N-terminalsearch Carbamoyl-phosphate synthase large subunit, CPSase domainsearch Carbamoyl-phosphate synthase, large subunitsearch Methylglyoxal synthase-like domainsearch ATP-grasp foldsearch ATP-grasp fold, subdomain 1search ATP-grasp fold, subdomain 2search Pre-ATP-grasp domainsearch
B, D, F, H Carbamoyl-phosphate synthase, small subunit N-terminal domainsearch Carbamoyl-phosphate synthase, small subunitsearch Glutamine amidotransferasesearch Class I glutamine amidotransferase-likesearch