› 1coy

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF CHOLESTEROL OXIDASE COMPLEXED WITH A STEROID SUBSTRATE. IMPLICATIONS FOR FAD DEPENDENT ALCOHOL OXIDASES

Released:
DOI: 10.2210/pdb1coy/pdb
PDB entry 1coy coloured by chain, front view.
PDB entry 1coy coloured by chain, side view.
PDB entry 1coy coloured by chain, top view.
Source organism: Brevibacterium sterolicum
Primary publication:
Crystal structure of cholesterol oxidase complexed with a steroid substrate: implications for flavin adenine dinucleotide dependent alcohol oxidases.
Li J, Vrielink A, Brick P, Blow DM
Biochemistry 32 11507-15 (1993)
PMID: 8218217

Function and Biology Details

Reactions catalysed: 
Cholesterol + O(2) = cholest-5-en-3-one + H(2)O(2)
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149748 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholesterol oxidase Chain: A
Molecule details ›
Chain: A
Length: 507 amino acids
Theoretical weight: 54.89 KDa
Source organism: Brevibacterium sterolicum
Expression system: Not provided
UniProt:
  • Canonical: P22637 (Residues: 46-552; Coverage: 100%)
Gene name: choB
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand AND 1 x AND
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 67.93Å b: 84.23Å c: 87.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.159 not available
Expression system: Not provided

Quick links

Citations

16 review citations

Comparative anatomy of the aldo-keto reductase superfamily.
Jez et al. (1997)
15 more

16 mentions without citation

Human choline dehydrogenase: medical promises and biochemical challenges.
Salvi et al. (2013)
15 more