PDBe Entry: 1cju

COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH BETA-L-2',3'-DIDEOXYATP AND MG

Summary

Header

LYASE/LYASE/SIGNALING PROTEIN

Method

X-RAY DIFFRACTION

Experiment

Resolution: 2.8 Å, R-factor: 22.2%, Free R-factor: 28.4%, Spacegroup: P 21 21 2

Released

31/08/1999, deposition: 16/04/1999, last revision: 24/02/2009

Authors

Tesmer, J.J.G.

; Sprang, S.R.

Primary citation

Two-metal-Ion catalysis in adenylyl cyclase.
SCIENCE

vol:285, pag:756-760 (1999) [PubMed ID 10427002 ]

Keywords

COMPLEX (LYASE/HYDROLASE)

, HYDROLASE

, SIGNAL TRANSDUCING PROTEIN

, CYCLASE

, EFFECTOR ENZYME

, LYASE/LYASE/SIGNALING PROTEIN COMPLEX

4.6.1.1 ExPASy BRENDA

(A B)

Organism

Canis lupus familiaris(dog) 9615

(A),Rattus norvegicus(Norway rat) 10116

(B),Bos taurus(cattle) 9913

(C)

UniProt

Adenylate cyclase type 5 (EC 4.6.1.1) (Adenylate cyclase type V) (ATP pyrophosphate-lyase 5) (Adenylyl cyclase 5) (Ca(2+)-inhibitable adenylyl cyclase) P30803

(A)
Adenylate cyclase type 2 (EC 4.6.1.1) (Adenylate cyclase type II) (ATP pyrophosphate-lyase 2) (Adenylyl cyclase 2) P26769

(B)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (Adenylate cyclase-stimulating G alpha protein) P04896

(C)

Solvent

A, B, C

Polymers

Id	Name	Type	UniProt	Residues	Observed
A	ADENYLATE CYCLASE, TYPE V	Protein	P30803 (ADCY5_CANFA)	217	87%
B	ADENYLATE CYCLASE, TYPE II	Protein	P26769 (ADCY2_RAT)	212	89%
C	GUANINE NUCLEOTIDE-BINDING PROTEIN G(S)	Protein	P04896 (GNAS2_BOVIN)	402	82%

Heterogens

Id	Name	Ligands
C, A	MAGNESIUM ION	MG
C	CHLORIDE ION	CL
C	5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE	GSP
A	FORSKOLIN	FOK
A	2',3'-DIDEOXYADENOSINE-5'-TRIPHOSPHATE	DAD