› 1cit

X-ray diffraction
2.7Å resolution

DNA-BINDING MECHANISM OF THE MONOMERIC ORPHAN NUCLEAR RECEPTOR NGFI-B

Released:
DOI: 10.2210/pdb1cit/pdb
PDB entry 1cit coloured by chain, front view.
PDB entry 1cit coloured by chain, side view.
PDB entry 1cit coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
DNA-binding mechanism of the monomeric orphan nuclear receptor NGFI-B.
Meinke G, Sigler PB
Nat Struct Biol 6 471-7 (1999)
PMID: 10331876

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113542 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Nuclear receptor subfamily 4 group A member 1 Chain: A
Molecule details ›
Chain: A
Length: 89 amino acids
Theoretical weight: 10.1 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22829 (Residues: 264-352; Coverage: 15%)
Gene names: Hmr, Ngfib, Nr4a1
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*CP*CP*GP*AP*AP*AP*AP*GP*GP*TP*CP*AP*TP*GP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.93 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
DNA (5'-D(*CP*GP*CP*AP*TP*GP*AP*CP*CP*TP*TP*TP*TP*CP*GP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.87 KDa
Source organism: Rattus norvegicus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P21
Unit cell:
a: 53.42Å b: 34.24Å c: 56.26Å
α: 90° β: 109.13° γ: 90°
R-values:
R R work R free
0.219 0.219 0.295
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

13 review citations

Regulation of cytochrome P450 (CYP) genes by nuclear receptors.
Honkakoski et al. (2000)
12 more

13 mentions without citation

Overview of protein structural and functional folds.
Sun et al. (2004)
12 more