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CRYSTAL STRUCTURE OF CITROBACTER FREUNDII RESTRICTION ENDONUCLEASE CFR10I AT 2.15 ANGSTROMS RESOLUTION.

X-ray diffraction
2.15Å resolution
PDB entry 1cfr coloured by chain, front view.
PDB entry 1cfr coloured by chain, side view.
PDB entry 1cfr coloured by chain, top view.
Source organism: Citrobacter freundii
Assembly composition:
homo tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Type II restriction enzyme Cfr10I
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Multimeric state: homo tetramer
Accessible surface area: 46209.6 Å2
Buried surface area: 9957.07 Å2
Dissociation area: 2,319.67 Å2
Dissociation energy (ΔGdiss): 19.94 kcal/mol
Dissociation entropy (TΔSdiss): 14.94 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-157420

Macromolecules

Type II restriction enzyme Cfr10I
Chain: A
Length: 285 amino acids
Theoretical weight: 32.05 KDa
Source organism: Citrobacter freundii
Expression system: Escherichia coli
UniProt:
  • Canonical: P56200 (Residues: 1-285; Coverage: 100%)
Gene name: cfr10IR
Pfam: Cfr10I/Bse634I restriction endonuclease
InterPro:
CATH: Restriction Endonuclease
SCOP: Cfr10I/Bse634I
Type II restriction enzyme Cfr10I in PDB entry 1cfr, assembly 1, front view.
Type II restriction enzyme Cfr10I in PDB entry 1cfr, assembly 1, side view.
Type II restriction enzyme Cfr10I in PDB entry 1cfr, assembly 1, top view.
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