1cc1 Summary

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CRYSTAL STRUCTURE OF A REDUCED, ACTIVE FORM OF THE NI-FE-SE HYDROGENASE FROM DESULFOMICROBIUM BACULATUM

The structure was published by Garcin, E., Vernede, X., Hatchikian, E.C., Volbeda, A., Frey, M., and Fontecilla-Camps, J.C., in 1999 in a paper entitled "The crystal structure of a reduced [NiFeSe] hydrogenase provides an image of the activated catalytic center" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.15 Å and deposited in 1999.

The experimental data on which the structure is based was not deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely HYDROGENASE (SMALL SUBUNIT) and HYDROGENASE (LARGE SUBUNIT).

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
S HYDROGENASE (SMALL SUBUNIT) P13063 (33-315) (PHSS_DESBA)search Desulfomicrobium baculatumsearch 100% 283 97%
L HYDROGENASE (LARGE SUBUNIT) P13065 (2-499) (PHSL_DESBA)search Desulfomicrobium baculatumsearch 97% 498 97%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P13063 (33 - 315) HYDROGENASE (SMALL SUBUNIT) Desulfomicrobium baculatum
P13065 (2 - 499) HYDROGENASE (LARGE SUBUNIT) Desulfomicrobium baculatum

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
S (P13063) Nickel-iron hydrogenase, small subunitsearch Rossmann foldsearch, Cytochrome-c3 Hydrogenase, chain A, domain 2search PF01058: NADH ubiquinone oxidoreductase, 20 Kd subunitsearch, PF14720: NiFe/NiFeSe hydrogenase small subunit C-terminalsearch
L (P13065) Nickel-iron hydrogenase, large subunitsearch Cytochrome-c3 Hydrogenase, chain Bsearch PF00374: Nickel-dependent hydrogenasesearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
S (P13063) 4 iron, 4 sulfur cluster bindingsearch oxidoreductase activitysearch iron-sulfur cluster bindingsearch metal ion bindingsearch ferredoxin hydrogenase activitysearch hydrogenase (acceptor) activitysearch oxidation-reduction processsearch ferredoxin hydrogenase complexsearch periplasmic spacesearch
L (P13065) nickel cation bindingsearch ferredoxin hydrogenase activitysearch hydrogenase (acceptor) activitysearch metal ion bindingsearch oxidoreductase activitysearch oxidation-reduction processsearch periplasmic spacesearch

Chain InterPro annotation
S [NiFe]-hydrogenase, small subunitsearch NADH:ubiquinone oxidoreductase-like, 20kDa subunitsearch Cytochrome-c3 hydrogenase, C-terminalsearch
L Nickel-dependent hydrogenase, large subunitsearch Nickel-dependent hydrogenase, large subunit, nickel binding sitesearch