Function and Biology Details
Reaction catalysed:
Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- Pancreatic trypsin inhibitor Kunitz domain
- Serine proteases, trypsin domain
- Pancreatic trypsin inhibitor Kunitz domain superfamily
- Peptidase S1A, chymotrypsin family
- Peptidase S1, PA clan, chymotrypsin-like fold
- Peptidase S1, PA clan
- Proteinase inhibitor I2, Kunitz, conserved site
- Serine proteases, trypsin family, serine active site
1 more domain
Structure domains:
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133407 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Chymotrypsin A chain A
Molecule details ›
Chains: A, F
Length: 13 amino acids
Theoretical weight: 1.25 KDa
Source organism: Bos taurus
UniProt:
Length: 13 amino acids
Theoretical weight: 1.25 KDa
Source organism: Bos taurus
UniProt:
- Canonical:
P00766 (Residues: 1-13; Coverage: 5%)
Chymotrypsin A chain B
Molecule details ›
Chains: B, G
Length: 131 amino acids
Theoretical weight: 13.93 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
Structure domains: Trypsin-like serine proteases
Length: 131 amino acids
Theoretical weight: 13.93 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
- Canonical: P00766 (Residues: 16-146; Coverage: 54%)
Structure domains: Trypsin-like serine proteases
Chymotrypsin A chain C
Molecule details ›
Chains: C, H
Length: 97 amino acids
Theoretical weight: 10.07 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
Structure domains: Trypsin-like serine proteases
Length: 97 amino acids
Theoretical weight: 10.07 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
- Canonical: P00766 (Residues: 149-245; Coverage: 40%)
Structure domains: Trypsin-like serine proteases
Soluble APP-beta
Molecule details ›
Chains: D, I
Length: 54 amino acids
Theoretical weight: 6.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain
Length: 54 amino acids
Theoretical weight: 6.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: P05067 (Residues: 289-342; Coverage: 7%)
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SSRL BEAMLINE BL7-1
Spacegroup:
P21212
Expression system: Escherichia coli
