› 1c7n

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF CYSTALYSIN FROM TREPONEMA DENTICOLA CONTAINS A PYRIDOXAL 5'-PHOSPHATE COFACTOR

Released:
DOI: 10.2210/pdb1c7n/pdb
PDB entry 1c7n coloured by chain, front view.
PDB entry 1c7n coloured by chain, side view.
PDB entry 1c7n coloured by chain, top view.
Source organism: Treponema denticola
Primary publication:
Crystal structure of cystalysin from Treponema denticola: a pyridoxal 5'-phosphate-dependent protein acting as a haemolytic enzyme.
Krupka HI, Huber R, Holt SC, Clausen T
EMBO J 19 3168-78 (2000)
PMID: 10880431

Function and Biology Details

Reaction catalysed: 
(1a) an L-cysteine-S-conjugate = a thiol + 2-aminoporp-2-enoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176343 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cysteine-S-conjugate beta-lyase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 399 amino acids
Theoretical weight: 46.28 KDa
Source organism: Treponema denticola
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56257 (Residues: 1-399; Coverage: 100%)
Gene name: hly
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 8 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P21
Unit cell:
a: 89.47Å b: 108.44Å c: 176.09Å
α: 90° β: 90.17° γ: 90°
R-values:
R R work R free
0.231 0.208 0.247
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia: the "red complex", a prototype polybacterial pathogenic consortium in periodontitis.
Holt et al. (2005)
8 more

7 mentions without citation

Crystal structure of cystalysin from Treponema denticola: a pyridoxal 5'-phosphate-dependent protein acting as a haemolytic enzyme.
Krupka et al. (2000)
6 more