› 1c04

X-ray diffraction
5Å resolution

IDENTIFICATION OF KNOWN PROTEIN AND RNA STRUCTURES IN A 5 A MAP OF THE LARGE RIBOSOMAL SUBUNIT FROM HALOARCULA MARISMORTUI

Released:
DOI: 10.2210/pdb1c04/pdb
PDB entry 1c04 coloured by chain, front view.
PDB entry 1c04 coloured by chain, side view.
PDB entry 1c04 coloured by chain, top view.
Source organism: Haloarcula marismortui
Primary publication:
Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit.
Ban N, Nissen P, Hansen J, Capel M, Moore PB, Steitz TA
Nature 400 841-7 (1999)
PMID: 10476961

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135755 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (6 distinct):
Large ribosomal subunit protein uL2 Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 14.93 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P04257 (Residues: 61-197; Coverage: 50%)
Gene name: rplB
Sequence domains:
Large ribosomal subunit protein uL6 Chain: B
Molecule details ›
Chain: B
Length: 177 amino acids
Theoretical weight: 19.2 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P02391 (Residues: 2-178; Coverage: 99%)
Gene name: rplF
Sequence domains: Ribosomal protein L6
Large ribosomal subunit protein uL11 Chain: C
Molecule details ›
Chain: C
Length: 67 amino acids
Theoretical weight: 7.12 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P56210 (Residues: 63-129; Coverage: 50%)
Gene name: rplK
Sequence domains: Ribosomal protein L11, RNA binding domain
Large ribosomal subunit protein uL14 Chain: D
Molecule details ›
Chain: D
Length: 122 amino acids
Theoretical weight: 13.37 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P04450 (Residues: 1-122; Coverage: 100%)
Gene name: rplN
Sequence domains: Ribosomal protein L14p/L23e
23S RRNA FRAGMENT Chain: E
Molecule details ›
Chain: E
Length: 58 nucleotides
Theoretical weight: 18.73 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
23S RRNA FRAGMENT Chain: F
Molecule details ›
Chain: F
Length: 29 nucleotides
Theoretical weight: 9.38 KDa

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B, NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 212Å b: 301.4Å c: 576.3Å
α: 90° β: 90° γ: 90°

Quick links

Citations

58 review citations

Structural insights into translational fidelity.
Ogle et al. (2005)
57 more

6 mentions without citation

Unique pathological changes in the pancreas of fulminant type 1 diabetes.
Kobayashi et al. (2020)
5 more