› 1bxn

X-ray diffraction
2.7Å resolution

THE CRYSTAL STRUCTURE OF RUBISCO FROM ALCALIGENES EUTROPHUS TO 2.7 ANGSTROMS.

Released:
DOI: 10.2210/pdb1bxn/pdb
PDB entry 1bxn coloured by chain, front view.
PDB entry 1bxn coloured by chain, side view.
PDB entry 1bxn coloured by chain, top view.
Source organism: Cupriavidus necator
Primary publication:
The crystal structure of rubisco from Alcaligenes eutrophus reveals a novel central eight-stranded beta-barrel formed by beta-strands from four subunits.
Hansen S, Vollan VB, Hough E, Andersen K
J Mol Biol 288 609-21 (1999)
PMID: 10329167

Function and Biology Details

Reaction catalysed: 
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140728 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribulose bisphosphate carboxylase large chain, chromosomal Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 485 amino acids
Theoretical weight: 53.7 KDa
Source organism: Cupriavidus necator
Expression system: Not provided
UniProt:
  • Canonical: P0C2C2 (Residues: 1-486; Coverage: 100%)
Gene names: cbbL, cbbL1, cbxLC, cfxLC
Sequence domains:
Structure domains:
Ribulose bisphosphate carboxylase small subunit, chromosomal Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 139 amino acids
Theoretical weight: 16.06 KDa
Source organism: Cupriavidus necator
Expression system: Not provided
UniProt:
  • Canonical: P09658 (Residues: 1-139; Coverage: 100%)
Gene names: cbbS, cbxSC, cfxSC, rbcS
Sequence domains: Ribulose bisphosphate carboxylase, small chain
Structure domains: Ribulose bisphosphate carboxylase, small subunit

Ligands and Environments

1 bound ligand:
Ligand PO4 8 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: P43212
Unit cell:
a: 112Å b: 112Å c: 402.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.266 0.266 0.322
Expression system: Not provided

Quick links

Citations

5 review citations

Rubisco: structure, regulatory interactions, and possibilities for a better enzyme.
Spreitzer et al. (2002)
4 more

18 mentions without citation

The Diverse AAA+ Machines that Repair Inhibited Rubisco Active Sites.
Mueller-Cajar O. (2017)
17 more